Automation System for the Flexible Sample Preparation for Quantification of Δ9-THC-D3, THC-OH and THC-COOH from Serum, Saliva and Urine

In the life sciences, automation solutions are primarily established in field of drug discovery. However, there is also an increasing need for automated medical diagnostics, e.g., determination vitamins, medication or abuse. While actual metrological highly today, necessary sample preparation processes still mainly carried out manually. laboratory, flexible required that can be used to determine different target substances matrices. A suitable system based on liquid handler was implemented. It has been tested and validated three cannabinoid metabolites blood, urine saliva. To extract Δ9-tetrahydrocannabinol-D3 (Δ9-THC-D3), 11-hydroxy-Δ9-tetrahydrocannabinol (THC-OH) 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH) from serum, saliva both rapidly cost-effectively, methods with a handling robot presented this article, basic framework which identical SPE method so they quickly exchanged against each other when matrix changed. If necessary, matrices could prepared parallel. For sensitive detection analytes, protein precipitation preparing serum before hydrolysis cleave glucuronide conjugate. Recoveries developed >77%. Coefficients variation <4%. LODs below 1 ng/mL comparison manual process shows significant cost reduction.